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. 2000 Nov 1;116(5):599–608. doi: 10.1085/jgp.116.5.599

Figure 4.

Figure 4

R201A mutants alter sensitivity to PIP2 and ATP. (A and C) Representative currents recorded from inside-out membrane patch containing Kir6.2[R201A] and Kir6.2[R206A] subunits coexpressed with SUR1 subunits. (B) Steady state dependence of membrane current on [ATP] (relative to current in zero ATP) for Kir6.2[R201A] or wild-type Kir6.2 subunits coexpressed with SUR1 (mean ± SEM, n = 5–7). The data were fitted using the Hill equation, with K1/2,ATP = 12 μM (WT) and 115 μM (R201A), and H = 1.9.(WT) and 0.9 (R201A).