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. 2000 Nov 1;116(5):623–636. doi: 10.1085/jgp.116.5.623

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The open conformation of the inactivation gate is destabilized by the E418C mutation and even more so by TMRM conjugation. (A) Ionic currents from a series of steps (−120 to +60 mV, increments of 10 mV) generate rapidly inactivating currents despite a high concentration (110 mM) of external K⁺. (B) Same oocyte as in A, with ionic currents blocked by 1 μM of AgTx-2, displays only gating currents. (C) Ionic currents are also eliminated (leaving only gating currents) by conjugation of 418C with TMRM, which is consistent with stabilization of the closed conformation of the inactivation gate.