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. 2000 Nov 1;116(5):721–734. doi: 10.1085/jgp.116.5.721

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Closed and open states of KCNKΔ299-1001 channels are similar to wild type. Single KCNKΔ299-1001 channels were studied as in Fig. 2 and Fig. 3. Open (O) and closed (C) state levels are indicated. (A) Sample 5-min recording of a single channel at 60 mV filtered at 20 Hz. (B) Expansion of the indicated portion of A, filtered at 3 kHz, showing interburst behavior. (C) Open time histogram from 25 s of recording during open bursts filtered at 3 kHz. (D) Closed time histograms: (left) obtained from 25 s of recording during open bursts filtered at 3 kHz; and (right) obtained from 23 min of single KCNKØ channel recording filtered at 100 Hz. Five single channel records were combined for this analysis.