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. 2000 Aug 1;116(2):299–310. doi: 10.1085/jgp.116.2.299

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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A representative recording showing the kinetics of the apical small-conductance K channel in the mouse CCD. (A) Experiment was carried out in a cell-attached patch with pipette solution (mM): 140 KCl, 1.8 MgCl₂, and 10 HEPES; and bath solution (mM): 140 NaCl, 5 KCl, 1.8 MgCl₂, 1.8 CaCl₂, and 10 HEPES. Different holding potentials (−Vp, from 40 to −60 mV) were applied and are indicated on right side of each trace. Channel closed and open states are indicated by C and O, respectively. (B) The channel open- and closed-time histograms. The mean closed time was 1.4 ± 0.01 ms and mean open time was 22.7 ± 0.03 ms. (C) I-V curve of the apical K channel. The slope conductance was 28.4 pS measured between −20 and 20 mV.