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. 2000 Aug 1;116(2):299–310. doi: 10.1085/jgp.116.2.299

Figure 5.

Figure 5

A tracing shows that extracellular ATP (100 μM) was applied into the pipette, which means ATP can only block the channel activity through apical membrane, indicating P2 receptors were located on the apical membrane of CCD. 100 μM cAMP was used to restore the channel activity to exclude the possibility of channel run down (n = 4).