TABLE V.
1 | 2 | 3 | 4 | 5 | 6 | 7 |
---|---|---|---|---|---|---|
Parameters and units | Measurements | 50 nM [Ca2+]R
2.5 pA × 4.6 ms |
100 nM [Ca2+]R
4.9 pA × 4.6 ms |
No Ca2+ pump 2.2 pA × 4.6 ms |
No ATP 1.7 pA × 4.6 ms |
Simplified fluo-3 1.3 pA × 4.6 ms |
Distance (D) (μm) | 0.405 ± 0.003 | 0.420 ± 0.003 | 0.430 ± 0.003 | 0.348 ± 0.002 | 0.350 ± 0.002 | |
0–100% risetime (ms) | 4.176 ± 0.034 (4.55) |
4.194 ± 0.023 (4.58) |
4.159 ± 0.023 (4.57) |
4.175 ± 0.023 (4.65) |
4.020 ± 0.021 (4.51) |
4.389 ± 0.026 (4.64) |
Peak amplitude (ΔF/F) | 1.082 ± 0.007 (0.97) |
1.077 ± 0.005 (1.04) |
1.081 ± 0.006 (1.04) |
1.083 ± 0.006 (1.02) |
1.079 ± 0.005 (1.07) |
1.083 ± 0.005 (1.11) |
Decay time constant (ms) | 4.601 ± 0.044 (5.19) |
5.747 ± 0.037 (5.72) |
5.981 ± 0.040 (5.91) |
4.706 ± 0.033 (4.70) |
4.933 ± 0.034 (4.88) |
7.270 ± 0.042 (7.04) |
FDHM (ms) | 5.923 ± 0.037 (6.56) |
6.820 ± 0.030 (6.98) |
7.044 ± 0.032 (7.13) |
6.012 ± 0.025 (6.30) |
6.103 ± 0.026 (6.33) |
8.097 ± 0.033 (7.94) |
Late offset (ΔF/F) | 0.0125 ± 0.0010 (0.014) |
0.0244 ± 0.0006 (0.025) |
0.0314 ± 0.0006 (0.033) |
0.0202 ± 0.0006 (0.021) |
0.0275 ± 0.0006 (0.029) |
0.0243 ± 0.0007 (0.024) |
FWHM (μm) | 1.019 ± 0.007 (0.96) |
1.014 ± 0.005 (0.97) |
1.040 ± 0.005 (1.00) |
1.067 ± 0.005 (1.03) |
0.855 ± 0.004 (0.83) |
0.852 ± 0.004 (0.80) |
Mass (μm3) | 1.938 ± 0.047 (1.03) |
1.528 ± 0.027 (1.14) |
1.646 ± 0.026 (1.24) |
1.784 ± 0.028 (1.37) |
0.947 ± 0.019 (0.73) |
0.922 ± 0.019 (0.69) |
Amplitude at 28–48 ms (ΔF/F) | (0.018) | (0.030) | (0.040) | (0.023) | (0.031) | (0.041) |