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. 2002 Sep;120(3):437–446. doi: 10.1085/jgp.20028621

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Representative currents recorded from inside-out membrane patches containing Kir6.2 WT, Kir6.2[R16A], or Kir6.2[R27A], coexpressed with SUR1. In this and subsequent figures, the patch was excised at the arrow, and the bars indicate the application of PIP₂ (5 μg/ml, unless indicated) or different [ATP] (as shown). Solutions were switched by moving the patch from one lane of the oil-gate chamber to another. The lower dashed line indicates zero current. I_initial and I_PIP₂, used to determine P_o,zero (see materials and methods), are indicated by additional dashed lines. The inserts in this and Fig. 3 show expanded records from the first 50–100 s after patch excision, from which ATP sensitivity and open probability (NA method) were estimated.