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. 2002 Oct;120(4):567–579. doi: 10.1085/jgp.20028665

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Copyright © 2002, The Rockefeller University Press

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Figure 9. — Effect of low pH_i on the size of the transient increases in V_Ca induced by either iontophoretic injections (♦) or 10 s depolarizations (▪). The calcium transients from each period of acid injection were normalized relative to the largest transient in that series, then collected in 0.2 pH unit bins (the pH at the start of each injection). The mean transient size and SEM for each bin was plotted against the average starting pH_i for the transients in each bin. Data from five different experiments, with a total of 26 injections and 25 depolarizations. Lines are least squares best fits to the original normalized data of the following relationship: pH_st = pK + log (ΔCa/1 − ΔCa), where pH_st is the pH_i at the start of a V_Ca transient, pK the pH when ΔCa = 0.5, and ΔCa the relative size of the calcium transient. The pKs giving the two lines were 6.05 for the injections and 6.51 for the depolarizations.