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. 2003 Aug;122(2):225–237. doi: 10.1085/jgp.200308822

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Copyright © 2003, The Rockefeller University Press

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Figure 4. — Open state stability is retained in the E229R/R314E double reverse mutant channels. (A) Representative currents recorded from inside-out membrane patches containing R192E, R301E, R314E, or E229R mutant channels. R301E channels did not give rise to measurable currents. All the other three mutant channels exhibited inactivation. (B) Currents recorded from membrane patches isolated from cells expressing the double charge reversal mutant E229R/R192E, E229R/R301E, or E229R/R314E. No currents were detected for the E229R/R301E channels. The E229R/R192E mutant channels still showed inactivation. In contrast, the E229R/R314E double mutant did not inactivate and behaved like WT channels.