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. 2003 Aug;122(2):225–237. doi: 10.1085/jgp.200308822

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Copyright © 2003, The Rockefeller University Press

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Figure 5. — Characterization of the E229C and the R314C mutant channels. (A) A representative current trace recorded from an inside-out membrane patch containing the E229C mutant channels. The channels displayed inactivation similar to that observed in the E229A and E229R channels upon patch excision into the K-INT/EDTA solution. Exposure to 1 mM ATP (indicated by black bars below the recordings) partially reactivated the channels, which again underwent rapid inactivation. (B) The R314C Kir6.2 mutant protein is expressed and incorporated into the K_ATP channel complex. Surface immunofluorescent staining of cells coexpressing FLAG-tagged SUR1 and either the WT or the R314C mutant Kir6.2. Immunostaining was performed in living cells at 4°C using the M2 anti-FLAG monoclonal antibody followed by Cy3-conjugated secondary antibody. Surface labeling of the FLAG-SUR1 was observed in both cells coexpressing the WT or the R314C mutant Kir6.2, demonstrating that the R314C Kir6.2 is properly incorporated into the K_ATP channel complex.