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. 2002 Dec;120(6):897–906. doi: 10.1085/jgp.20028608

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Current-voltage relationships for the benzamil-sensitive pathway in the apical membrane. (A) Experiments were performed using monolayers cultured in serum-free media with insulin for 3 d. Benzamil-sensitive difference currents were obtained using a voltage step protocol that ranged from −100 to 95 mV in 15-mV increments from a holding potential of 0 mV. Benzamil (5 μM) was added apically to either permeabilized control cell monolayers (top trace) or to permeabilized monolayers activated with 5 μM UTP (bottom trace). (B) Benzamil-sensitive current-voltage relationships were obtained in response to a voltage step protocol from −90 to 90 mV in 15-mV increments from a holding potential of 0 mV. Benzamil (5 μM) was added to the apical solution in the absence or presence of UTP (1 μM).