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. 2002 Dec;120(6):897–906. doi: 10.1085/jgp.20028608

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Copyright © 2002, The Rockefeller University Press

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Figure 9. — Effect of BAPTA-AM on changes in [Ca² ⁺]_i in response to UTP. (A) Temporal changes in [Ca²⁺]_i in response to UTP were determined using fura 2-AM as described in materials and methods. Upon stimulation with 5 μM UTP in Ca²⁺-containing HBSS, control cells initially exhibited a transient elevation in [Ca²⁺]_i, followed by a gradual decline to a new sustained elevated [Ca²⁺]_i. Removing UTP from the bathing solution returned the intracellular calcium concentrations back to baseline levels. (B) BAPTA-AM reduced the basal level of intracellular calcium by 80 nM, and upon stimulation with UTP, [Ca²⁺]_i increased by only 20 nM. For this figure, measurements were obtained from 20 cells in each of three separate experiments.