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. 2002 Dec;120(6):875–885. doi: 10.1085/jgp.20028695

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Sustained spontaneous ciliary activity requires intracellular ATP. CBF measurements as a function of time, following the establishment of the whole-cell configuration of the patch clamp technique (time zero). CBF measurements from two different cells are presented. In A, the intracellular (pipette) solution contained (in mM): CsCl 155, EGTA 1, TES 5, adjusted to pH 7.2 with CsOH. In B, the pipette solution contained (in mM): CsCl 148, EGTA 1, TES 5, MgATP 5, K₂ATP 0.5, adjusted to pH 7.2 with CsOH. Ca²⁺ was adjusted to 100 nM in both solutions.