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. 2003 Nov;122(5):541–556. doi: 10.1085/jgp.200308905

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Copyright © 2003, The Rockefeller University Press

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Figure 10. — Single-channel properties of the Shaker P475Q mutant channel recorded in symmetrical K⁺. (A) Representative current traces (left) and corresponding all points amplitude histograms (right) recorded in symmetrical 140 mM KCl. The single channel patch shown was held at −100 mV and stepped to −150 mV for 350 ms before the traces shown. Filter frequency was 2 kHz. Horizontal dashed lines correspond to the zero current level. Y-axes for histograms are number of events and each histogram contains data from 6–10 sweeps. For inset histograms, y-axes range from 0 to (from top to bottom) 10³, 5 × 10², and 10³. (B) Ensemble of 30 single-channel sweeps from the same patch shown in A and elicited using the same protocol as in A. (C) i-V relationship for the highest conducting state. Each data point represents the mean ± SD (n = 6). Linear regression of the data shown yields a conductance of 60 pS. (D) Probability (P) for the closed and highest conducting open state as a function of voltage. All point histograms in A were fit with multiple Gaussians and their relative areas used to estimate probabilities.