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. 2003 Dec;122(6):749–760. doi: 10.1085/jgp.200308823

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Copyright © 2003, The Rockefeller University Press

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Figure 9. — Voltage dependence of PsTx dissociation from CNGA2 and interaction with permeant ions. (A) Dissociation of PsTx at holding potentials of −60 and +60 mV in the presence of symmetrical NaCl. Smooth red lines indicate single exponential fits with time constants of 67 s (−60 mV) and 7 s (+60 mV). (B) Dissociation of PsTx at holding potentials of −60 and +60 mV under asymmetrical conditions (13 mM NaCl; 117 NMDG-Cl in the pipette solution). Smooth red lines indicate single exponential fits with time constants of 20 s (−60 mV) and 10 s (+60 mV). Solutions changes are indicated by arrows. C, control solution; P, 500 nM PsTx; M, 10 mM MgCl₂.