Table 1.
The Influence of holding Potential on the Interaction of Ryanodol and 21-Amino-9α-Hydroxyryanodine with Sheep RYR2
| Ryanodol | 21-Amino-9α-Hydroxyryanodine | |
|---|---|---|
| Slope (z on F/RT) | 0.041 ± 0.003 (r = 1.0) | 0.051 ± 0.002 (r = 1.0) |
| z on | 1.03 | 1.29 |
| Slope (−z on F/RT) | −0.019 ± 0.002 (r = 1.0) | −0.0344 ± 0.002 (r = 1.0) |
| z off | 0.48 | 0.87 |
| z total | 1.51 | 2.16 |
| k on at 0 mV (μM−1 s−1) | 0.035 | 0.365 |
| k off at 0 mV (s−1) | 0.095 | 0.990 |
| K d at 0 mV (μM) | 2.81 | 2.79 |
Data for 21-amino-9α-hydroxyryanodine are from Tanna et al. 1998 with values of k on normalized for a P o of 1.0. Values of k on, k off, and K d at 0 mV are calculated from the regression lines fitted to data in Fig. 4 and Fig. 5.