Figure 5.

Effects of IN3 on plasma membrane expression of HA-GnRHRs in MCF7 and DU145 cells. MCF7 (upper panels) and DU145 (lower panels) cells infected with Ad expressing the indicated GnRHRs and incubated for 16 h in medium with 0 (ctrl., open bars) or 1·8×10⁻⁷ M IN3 (filled bars) before imaging of cell surface and whole cell receptors. The left panels show expression indices and the right panels show PCSE values, pooled from four separate experiments each with three or four replicates (mean±s.e.m., n=4). Two-way ANOVAs revealed IN3 as a significant variable (P<0·05) for each receptor and each endpoint, and the effects of IN3 on PCSE were statistically significant (*P<0·05, **P<0·01 when compared with control values matched for cell and receptor type) for both receptors and both cell types.