Figure 9.
Effects of membrane-permeant and membrane-impermeant ligands on plasma membrane expression of HA-GnRHRs. MCF7 cells were infected with Ad expressing HA-hGnRHRs (upper panel) or h.XGnRHRs (lower panel) and then incubated for 16 h with control medium (ctrl.) or medium supplemented with IN3 (1·8×10−6 M), antide (ant.; 10−8 M), cetrorelix (cet.; 10−8 M), or GnRH (G, 10−8 M) before imaging. The data shown are PCSE values pooled from seven separate experiments each with three or four replicates (mean±s.e.m., n=4–7) and the effects of IN3 were statistically significant at both receptors, whereas the effects of antide and cetrorelix were significant only at the HA-h.XGnRHRs (*P<0·05, **P<0·01 compared with control values for receptor type).