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. Author manuscript; available in PMC: 2008 Feb 5.
Published in final edited form as: Am J Physiol Heart Circ Physiol. 2007 Jul 27;293(4):H2210–H2218. doi: 10.1152/ajpheart.00688.2007

Fig. 1.

Fig. 1

Cannabinoid-1 (CB1) and cannabinoid-2 (CB2) receptors are expressed in human coronary artery endothelial cells (HCAECs). A: expression of CB1 and CB2 receptors in HCAECs demonstrated by immunofluorescence staining. CB1 expression and CB2 expression in the endothelial cells were detected with rabbit polyclonal anti-CB1 and anti-CB2 antibodies (Cayman Chemical) and detected by secondary antibody goat anti-rabbit FITC (Pierce); the nucleus was counterstained with 4′,6-diamidino-2-phenylindole (Molecular Probes, Invitrogen). B and C: RT-PCR analysis of CB1 (B) and CB2 (C) receptor expression in endothelial cells. D and E, left: Western immunoblot demonstrating expression of CB1 (D) and CB2 (E) in endothelial cells. Six samples from 3–5 passages from HCAECs or THP-1 and lysates from six brain tissues from wild-type C57Bl/6J mice were used to evaluate CB1 and CB2 expression in human endothelial cells. D and E, right: the quantification of CB1 and CB2 expression in HCAECs; n = 6 samples. *P < 0.05 vs. brain extract or THP-1 cells.