Figure 1.
Schematic representation of strain Toledo and Toledo-derived mutant virus genomes. (A, upper line) CMV strain Toledo genome structure with the invertable L and S genome segments indicated by arrows and inverted repeats (ab-b′a′c′-ca) indicated by boxes. Expanded region is the ULb′ region, with ORFs depicted as arrows. (B) Structures of the recombinant viruses. Nucleotide numbers correspond to Toledo sequences (accession no. U33331). (C) Sequence comparison between UL146 (Toledo), UL146 (Towne), UL147, and human IL-8. Identities are boxed, and the two most likely signal peptide cleavage sites predicted by computer algorithm are marked (Y18 and T23). The cleavage site of mature IL-8 and FLAG insertion site in UL146 (Toledo) also are indicated.