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Equilibrium-binding competition profiles of vCXC-1 on human PBN and individual CXC chemokine receptor-bearing cells. Unlabeled chemokines were incubated along with 0.1 nM 125I-IL-8 by using primary human neutrophils (A) or transfected murine NSO cells stably expressing human chemokine receptors CXCR1 (B) or CXCR2 (C). (D) Competition constants (Ki) for each interaction as derived from Scatchard analysis of the data.
