Skip to main content
PMC home page
The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1956 Jul 25;2(4):395–402. doi: 10.1083/jcb.2.4.395

STUDIES ON THE GOLGI APPARATUS BY ELECTRON MICROSCOPY WITH PARTICULAR REFERENCE TO AOYAMA'S TECHNIQUE

Dennis Lacy 1, Cyril E Challice 1
PMCID: PMC2229731  PMID: 13357503

Abstract

1. Aoyama's silver impregnation method for the Golgi apparatus has been used on exocrine cells of the pancreas of the mouse and studied by electron microscopy in order to determine as precisely as possible where the silver is deposited. Similar cells have also been fixed in buffered osmium tetroxide solution and compared with cells treated by the silver technique. 2. Examination of the Aoyama preparations usually revealed a light deposition of silver in the cytoplasm (hyaloplasm or matrix) and a heavy deposition of silver around a series of closely apposed vacuoles. The heavy deposition of silver was regarded as revealing the chromophilic region of the Golgi apparatus while the vacuoles were identified as the chromophobic component. 3. Comparison of the silver preparations with those fixed in buffered osmium tetroxide solution showed that the silver was primarily deposited in the region of the Golgi membranes.

Full Text

The Full Text of this article is available as a PDF (681.1 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAKER J. R. The expressions Golgi apparatus, Golgi body and Golgi substance. Nature. 1953 Oct 3;172(4379):617–618. doi: 10.1038/172617b0. [DOI] [PubMed] [Google Scholar]
  2. BAKER J. R. What is the Golgi controversy? J R Microsc Soc. 1954 Sep-Dec;74(3-4):217–221. doi: 10.1111/j.1365-2818.1954.tb01007.x. [DOI] [PubMed] [Google Scholar]
  3. BERNHARD W., GAUTIER A., ROUILLER C. La notion de microsomes et le problème de la basophilie cytoplasmique; étude critique et expérimentale. Arch Anat Microsc Morphol Exp. 1954;43(3):236–275. [PubMed] [Google Scholar]
  4. BURGOS M. H., FAWCETT D. W. Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J Biophys Biochem Cytol. 1955 Jul 25;1(4):287–300. doi: 10.1083/jcb.1.4.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DALTON A. J. A study of the golgi material of hepatic and intestinal epithelial cells with the electron microscope. Z Zellforsch Mikrosk Anat. 1952;36(6):522–524. doi: 10.1007/BF00347683. [DOI] [PubMed] [Google Scholar]
  6. DALTON A. J., FELIX M. D. Cytologic and cytochemical characteristics of the Golgi substance of epithelial cells of the epididymis in situ, in homogenates and after isolation. Am J Anat. 1954 Mar;94(2):171–207. doi: 10.1002/aja.1000940202. [DOI] [PubMed] [Google Scholar]
  7. DALTON A. J., FELIX M. D. Studies on the Golgi substance of the epithelial cells of the epididymis and duodenum of the mouse. Am J Anat. 1953 Mar;92(2):277–305. doi: 10.1002/aja.1000920204. [DOI] [PubMed] [Google Scholar]
  8. GATENBY J. B., MOUSSA T. A. A. The neurone of the human autonomic system and the so-called 'senility pigment'. J Physiol. 1951 Jun;114(1-2):252–254. doi: 10.1113/jphysiol.1951.sp004616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GATENBY J. B. The Golgi apparatus of the living sympathetic ganglion cell of the mouse, photographed by phase-contrast microscopy. J R Microsc Soc. 1953 Oct;73(2):61–68. doi: 10.1111/j.1365-2818.1953.tb02349.x. [DOI] [PubMed] [Google Scholar]
  10. GATENBY J. B. The Golgi apparatus. J R Microsc Soc. 1954 Sep-Dec;74(3-4):134–161. doi: 10.1111/j.1365-2818.1954.tb01001.x. [DOI] [PubMed] [Google Scholar]
  11. LACY D. Recent studies on the Golgi apparatus of the exocrine and endocrine cells of the mammalian pancreas and the cytoplasmic inclusions of other cells. J R Microsc Soc. 1954 Sep-Dec;74(3-4):226–240. doi: 10.1111/j.1365-2818.1954.tb01009.x. [DOI] [PubMed] [Google Scholar]
  12. LACY D. The Golgi apparatus and neutral-red granules in living and frozen dried endocrine cells of the pancreas of the mouse. J R Microsc Soc. 1954 Jun;74(1):1–9. doi: 10.1111/j.1365-2818.1954.tb01997.x. [DOI] [PubMed] [Google Scholar]
  13. LACY D. The Golgi apparatus of the exocrine and endocrine cells of the mouse pancreas. J R Microsc Soc. 1954 Apr;73(4):179–200. doi: 10.1111/j.1365-2818.1953.tb01988.x. [DOI] [PubMed] [Google Scholar]
  14. MOUSSA T. A. The cytoplasmic inclusions of the sympathetic neurons of the mouse. Am J Anat. 1952 May;90(3):379–425. doi: 10.1002/aja.1000900304. [DOI] [PubMed] [Google Scholar]
  15. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
  17. PALADE G. E., CLAUDE A. The nature of the Golgi apparatus; identification of the Golgi apparatus with a complex of myelin figures. J Morphol. 1949 Jul;85(1):71–111. doi: 10.1002/jmor.1050850104. [DOI] [PubMed] [Google Scholar]
  18. PALADE G. E., CLAUDE A. The nature of the Golgi apparatus; parallelism between intercellular myelin figures and Golgi apparatus in somatic cells. J Morphol. 1949 Jul;85(1):35–69. doi: 10.1002/jmor.1050850103. [DOI] [PubMed] [Google Scholar]
  19. PALAY S. L., PALADE G. E. The fine structure of neurons. J Biophys Biochem Cytol. 1955 Jan;1(1):69–88. doi: 10.1083/jcb.1.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PORTER K. R. Observations on a submicroscopic basophilic component of cytoplasm. J Exp Med. 1953 May;97(5):727–750. doi: 10.1084/jem.97.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. RINEHART J. F., FARQUHAR M. G. Electron microscopic studies of the anterior pituitary gland. J Histochem Cytochem. 1953 Mar;1(2):93–113. doi: 10.1177/1.2.93. [DOI] [PubMed] [Google Scholar]
  22. SJOSTRAND F. S., HANZON V. Ultrastructure of Golgi apparatus of exocrine cells of mouse pancreas. Exp Cell Res. 1954 Nov;7(2):415–429. doi: 10.1016/s0014-4827(54)80087-5. [DOI] [PubMed] [Google Scholar]
  23. WEISS J. M. The ergastoplasm; its fine structure and relation to protein synthesis as studied with the electron microscope in the pancreas of the Swiss albino mouse. J Exp Med. 1953 Dec;98(6):607–618. doi: 10.1084/jem.98.6.607. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press

RESOURCES