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. 1999 Aug 17;96(17):9845–9850. doi: 10.1073/pnas.96.17.9845

Figure 4.

Figure 4

Structural and functional conservation of sopE loci from S. typhimurium isolates. (a) Southern blot analysis of EcoRV-digested chromosomal DNA using probe V (see Materials and Methods). (b) PFGE–Southern analysis of XbaI-digested chromosomal DNA using probe VI. (c) Western blot analysis of SopE secretion using an α-SopE antiserum (IM1; Materials and Methods). Isolates shown in a– c: 1, 839/88DT49;E+; 2, 408/88DT49;E+; 3, 11635/98DT68;E+; 4, 1/82DT175;E+; 5, 1646/82DT175;E+; 6, 3351/78DT204;E+; 7, 93/80DT204;E+; 8, 1690/75DT204;E+; 9, 74/80DT204;E+; 10, 646/96DT204c;E+; 11, 6203/97DT204c;E+; 12, SARA4DT20;E+; 13, M1063351/78;aphT-Φ; and 14, 2728/96DT104;E−.