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. 2007 Dec 11;67(2):403–419. doi: 10.1111/j.1365-2958.2007.06050.x

Fig. 7.

Fig. 7

Assay of the GAG dependency of binding by C. caviae OmcB and C. trachomatis OmcB variants. Schematic representation of the adhesion of various yeast transformants to HEp-2 cells. Yeast cells expressing Aga2, Aga2–OmcB-C.pn., Aga2–OmcB-C.ca. GPIC, Aga2–OmcB-C.tr. L1, Aga2–OmcB-C.tr. E, Aga2–OmcB-C.tr. L1 P66L or Aga2–OmcB-C.tr. E L66P were pre-incubated with 500 μg ml−1 soluble heparin prior to the adhesion assay. (n = 1000 HEp-2 cells, No. experiments = 4, P < 0.0001). w/o, without; C.pn., C. pneumoniae; C.ca., C. caviae; C.tr., C. trachomatis.