Table 1.
Bacterial strains and plasmid used in this study
| Strain | Relevant genotype | Ref. or source |
|---|---|---|
| E. coli | ||
| AW405 | Wild type | 10 |
| RP437 | Wild type | 11 |
| HCB5 | AW405, fliC | 12 |
| HCB8 | AW405, fliK | This study |
| HCB626 | RP437, ΔcheY, λcI857(ts)[plac-cheY] | D. F. Blair |
| MS5037 | AW405, motA | 13 |
| S. typhimurium | ||
| SJW1103 | Wild type (f2) | 14 |
| SJW1655 | SJW1103, fliCA449V (f11) | 15 |
| SJW1660 | SJW1103, fliCG426A (f0) | 15 |
| SJW1664 | SJW1103, fliCG426A, A449T (f1) | 15 |
| SJW2868 | SJW1103, fliCA427V (f5/6*) | 16 |
| HCB1280 | SJW1660, ΔcheY | This study |
| HCB1281 | SJW1660, fliGΔ169-171 (ΔPAA) | This study |
| Plasmid | ||
| pDFB36 | plac-motA | 17 |
*
This allele is curly, but whether it is f5 or f6 has not been determined.