Figure 2.

CoREST interacts with the C-terminal repressor domain of REST. (a) The family of REST cDNAs used in in vitro and in vivo experiments with CoREST. The larger solid rectangle depicts the eight zinc finger motifs in the DBD; the smaller rectangle depicts the single zinc finger in the C terminus. REST (amino acids 1–1097) represents full-length protein. (b) Yeast two-hybrid interactions between pGADCoREST (amino acids 109–293) and four different REST-LexA fusion proteins. (c) REST/CoREST interactions in GST pull-down assay. Indicated REST constructs were transcribed and translated in vitro to yield ³⁵S-labeled products and were incubated with immobilized GST or GSTCoREST (amino acids 109–293). Input (3% of total protein) and proteins bound to GST were analyzed by SDS/PAGE.