Figure 3.

(a) CoREST is expressed in neuronal and nonneuronal cells. Western blot of nuclear extracts (50 μg) prepared from neuronal (PC12, GH3) and nonneuronal (HeLa, COS-1, L6) cell lines. The blot was probed with αCoREST antibody. Endogenous CoREST protein migrates at 66 kDa. (b) Demonstration of REST/CoREST interaction in vivo by coimmunoprecipitation analysis. Whole-cell lysates prepared from L6 skeletal muscle cells were immunoprecipitated with the indicated antibodies and then subjected to Western blotting. The membrane was probed with αREST antibody (1). The arrow depicts migration of REST protein. Input corresponds to 50 μg of extract. αCoREST and αGal4DBD refer to antibodies against CoREST and against the DBD of Gal4 protein, respectively.