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. 1999 Aug 17;96(17):9879–9884. doi: 10.1073/pnas.96.17.9879

Figure 1.

Figure 1

IGF-I promotes survival (solid line) and antiphosphotyrosine-precipitable PI3-kinase activity (dotted line) of primary murine cerebellar neurons. Five-day-old cerebellar granule neurons were washed, cultured without serum for 24 h, and treated with increasing concentrations of IGF-I for 1 day. Neuronal survival was determined with the mitochondrial dye 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (23). At 10 ng/ml, IGF-I tended (mean ± SEM; P < 0.10) to increase survival of primary neurons. Neuronal survival was significantly increased with doses of 50–200 ng/ml of IGF-I (∗, P < 0.005; n = 3). Treatment with IGF-I for 5 min increased the activity of PI3-kinase at concentrations greater than 10 ng/ml (∗, P < 0.05; n = 3).