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. 1999 Aug 17;96(17):9879–9884. doi: 10.1073/pnas.96.17.9879

Figure 3.

Figure 3

IRS-2 is the primary docking molecule expressed in murine cerebellar granule neurons. One hundred micrograms of whole-cell lysates from neurons was electrophoresed and blotted with specific antibodies to either IRS-1 or IRS-2. As a positive control, FDCP myeloid progenitor cells were used that were maintained in recombinant murine IL-3 (0.25 units/ml) and 5% heat-inactivated horse serum (36). In this representative Western blot, we could barely detect IRS-1 protein in cerebellar granule neurons, although the 165-kDa IRS-1 protein was easily visible in lysates from FDCP cells. In contrast, the 185-kDa IRS-2 protein was easily detectable in the same whole-cell lysates from either cerebellar granule neurons or FDCP cells.