Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Nov 1;114(5):637–652. doi: 10.1085/jgp.114.5.637

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Kinetic Scheme for Type II Na^+/P_i Cotransport. States occupied in the cis (outward facing) orientation are 1–5. States occupied in the trans (inward facing) orientation are 6–10. The three modes of operation that involve transmembrane reorientation, as revealed by steady state and pre–steady state studies of WT, are indicated by dark shading: empty carrier (10 ⇔ 1), slippage (2 ⇔ 9), and cotransport (5 ⇔ 6). At least two voltage-dependent transitions have been identified: empty carrier (10 ⇔ 1) and first external Na⁺ binding/release step (1 ⇔ 2). The voltage dependence of the last Na⁺ binding/release step on the trans side (9 ⇔ 10) has not been characterized. PFA binding places the system in state 2*, which when occupied prevents the slippage and cotransport modes. The lightly shaded region indicates those transitions and associated states that have been shown to remain intact after alkylation. In addition, the zero voltage rate constants for the empty carrier mode increase after alkylation (see text).