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T-DNA of the activation-tagging Ac-Ds vector pSQ5. RB and LB, Right and left borders of the T-DNA; Ubi, maize ubiquitin 1 promoter; GFP, GFP gene as a negative selection marker; RFP, RFP (or DsRed) gene as selection marker for the Ds element; 4xEn, a tetramer of CaMV 35S enhancers; HPH, hygromycin phosphotransferase gene as plant transformation selection marker. The T-DNA is in the backbone of pCAMBIA-1300.
