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. 2008 Jan;146(1):60–73. doi: 10.1104/pp.107.107409

Figure 7.

Figure 7.

Heterologous expression (A and B) and functional characterization (C–H) of BIO3-BIO1 gene products in E. coli. A and B, Protein extracts from isopropylthio-β-galactoside-induced E. coli cultures harboring either the empty vector (pDEST17) or the BIO3-BIO1 bicistronic (+10) or monocistronic (−10) full-length cDNA were subjected to SDS-PAGE and stained with Coomassie Blue. Putative BIO3 (A) and fusion (B) proteins are marked with arrows. C and D, Expected responses of wild-type (WT) and mutant strains of E. coli in the presence and absence of biotin. E and F, Responses of a wild-type control strain and bioD (E) and bioA (F) mutants transformed with either the pDEST17 (empty) vector or recombinant vectors containing the (−10) or (+10) cDNA. G and H, Responses of wild-type and transformed bioD (G) and bioA (H) strains in liquid cultures. E to H, Strains were lysogenized with λDE3 and cultured on a kanamycin medium without biotin.