Table V.
Response of mutant embryos cultured on DAPAa
| Genotype | Embryos Cultured | Extent of Embryo Response in Culture
|
||||
|---|---|---|---|---|---|---|
| A | B | C | D | F | ||
| bio1-1 | 60 | 44 | 6 | 10 | 0 | 0 |
| bio1-2 | 60 | 36 | 8 | 12 | 0 | 4 |
| bio3-3 | 60 | 0 | 0 | 1 | 52 | 7 |
| bio2-1 | 20 | 0 | 0 | 0 | 0 | 20 |
| Wild type | 40 | 25 | 5 | 2 | 8 | 0 |
a
Embryos were removed from immature siliques of heterozygotes and cultured on 1 to 2 μm DAPA. Embryo stages were noted at the time of culture. Responses were ranked after 21 d: A, extensive green callus with shoots; B, green callus with small shoots; C, green callus without shoots; D, trace amount of callus (unpigmented); F, no change in culture.