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. 2008 Feb;19(2):735–744. doi: 10.1091/mbc.E07-09-0968

Figure 2.

Figure 2.

Elimination of Arx1 impairs nuclear export of Nmd3 and 60S subunits in the nup120ΔC16 strain. (A) Functional analysis of regulatable Arx1-myc constructs. arx1Δ nup120ΔC16 cells expressing either wild-type Arx1-myc (pAJ1026), Gal-UBI(M)-Arx1-myc (pAJ1481) or Gal-UBI(R)-Arx1-myc (pAJ1484) plasmid as the sole copy of Arx1 were tested for growth on galactose- or glucose-containing dropout medium. (B) Protein expression of regulatable Arx1-myc expression constructs. The arx1Δ nup120ΔC16 cells harboring either Arx1-myc, Gal-UBI(M)-Arx1-myc (pAJ1481) or Gal-UBI(R)-Arx1-myc (pAJ1484) plasmid were cultured in galactose-containing media to midlog phase and transferred to glucose-containing media for the indicated times before they were collected. Extracts were subjected to SDS-PAGE and Western blotting using anti-myc antibody. (C) The arx1Δ nup120ΔC16 stain carrying the unstable Gal-UBI(R)-Arx1-myc construct was transformed with either Rpl25-eGFP (pAJ908) or Nmd3-GFP (pAJ755) plasmid. The strains were then cultured in galactose containing media to midlog phase and transferred to glucose containing media for the indicated times before they were subjected to microscopy. Nuclear DNA was stained with DAPI.