Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Mex67 and Mtr2 are enriched on pre-60S particles when export is blocked. Cell extracts were prepared from wild-type (BY4741) and arx1Δ (AJY1901) expressing Nmd3-myc (pAJ538) and Nmd3(AAA)-myc (pAJ752). The myc-tagged Nmd3 proteins were immunoprecipitated¤ and proteins that copurifying proteins were detected by Western blotting using antibodies against Mex67, Mtr2, Nmd3-myc, and Rpl8, as a marker for 60S subunits.
