Figure 5.

rhSOSTDC1 antagonizes the production of phosphorylated Smad in response to BMP-7 signaling. 786-O cells were treated BMP-7, SOSTDC1, or noggin as indicated and Smad phosphorylation was analyzed by Western blotting. SOSTDC1 inhibited the production of p-Smad-1, -5, and -8 by 3.5-fold. GAPDH was used as a loading control. Note: for clarity, lanes from the same blot have been rearranged. Similar results were observed in the 769-P and A-704 renal adenocarcinoma cell lines (data not shown).