Figure 1.

Phagocytic and OVAp-loaded macrophages induce CD69 and CD25 expression in T lymphocytes. CD8+ cells were analyzed for the surface expression of CD69 or CD25 molecules by flow cytometry. Dose-response assays of CD69 (A) and CD25 expression (B) determined as the percentage of positive cells. (C and D) Same as in A and B but granulocyte-depleted macrophages and naïve CD8+ T-cells were used. Macrophages without antigen (No Ag). T-cells without macrophages (No MF). (A–D) Data are expressed as arithmetic means ± SEM of four independent experiments; statistical comparisons were by the Mann-Whitney test (*p < 0.05). (E) Histograms of CD69 expression in CD8+ cells are shown from a representative experiment.