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. 2008 Feb;19(2):722–734. doi: 10.1091/mbc.E07-07-0662

Figure 10.

Figure 10.

Secretion defects are rescued upon reintroduction of Munc18-1 by transfection. (A) Immunoblot analysis of transfected Munc18-1 in the knockdown (KD43) cells. Thirty micrograms of total homogenates from KD43 cells transfected with the empty plasmid (control) or the Munc18-1 expression plasmid (Munc18-1(SNM)) were analyzed by SDS-PAGE and immunoblotting using anti-Munc18-1. (B) Secretion of NPY-hPLAP from the knockdown (KD43) cells that were transfected with the control plasmid or the Munc18-1 expression plasmid. *p < 0.01; a statistically significant difference. Error bar, SEM (n = 9). (C) Secretion of NPY-hPLAP from the control (C1) cells that were transfected with the control plasmid or the Munc18-1 expression plasmid. Error bar, SEM (n = 9).