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. 2008 Feb;19(2):722–734. doi: 10.1091/mbc.E07-07-0662

Figure 9.

Figure 9.

Rescue of Syntaxin1 localization upon reintroduction of Munc18-1 into KD43 cells. (A and B) Representative single-cell confocal images of KD43 cells that were transfected with pCMV-Munc18-1(SNM)-IRES-EGFP (A) as well as untransfected KD43 cells (B), both stained for anti-syntaxin1 antibodies followed by red-x–conjugated anti-mouse antibodies. Transfected cells were identified through the expression of IRES-EGFP. Scale bar, 5 μm. (C) Summarized figure shows the proportion of syntaxin1 found in the plasma membrane to that found inside the cell for KD43 cells transfected with pCMV-Munc18-1(SNM)-IRES-EGFP as well as untransfected KD43 cells. Error bar, SEM (n = 16). *p < 0.01; a statistically significant difference. (D) Confocal images of NGF-differentiated multiple KD43 cells transfected with pCMV-Munc18-1(SNM)-IRES-EGFP that were stained with anti-syntaxin1 monoclonal antibodies followed by red-x–conjugated goat anti-mouse antibodies. (E) Confocal images of NGF-differentiated KD43 cells transfected with pCMV-Munc18-1(SNM)-Emerald GFP that were stained with anti-syntaxin1 monoclonal antibodies followed by red-x–conjugated goat anti-mouse antibodies. Scale bar, 10 μm.