Table 2.
ESCRT-III requirements for localization of Ist1 and weak class E proteins
| Ist1-GFP | GFP-Did2 | GFP-Vta1 | Mvb12-GFP | |
|---|---|---|---|---|
| WT | Perivacuolar punctae and cytoplasm | Perivacuolar punctae | Perivacuolar punctae and cytoplasm | Perivacuolar punctae and cytoplasm |
| vps4Δ | E dot | E dot | E dot and cytoplasm | E dot |
| vps4Δ vps2Δ | Cytoplasm | Cytoplasm | Cytoplasm | E dot |
| vps4Δ vps24Δ | Cytoplasm | Cytoplasm | Cytoplasm | E dot |
| vps4Δ snf7Δ | E dot | E dot and cytoplasm | E dot and cytoplasm | E dot |
| vps4Δ vps20Δ | E dot | E dot and cytoplasm | E dot and cytoplasm | E dot |