Figure 4.

Coassembly and cell surface expression of θ with other GABA-A receptor subunits. c-myc epitope tagged θ (A) or c-myc-epitope-tagged β2 (B) was transiently transfected into HEK 293 cells, either alone or with other GABA-A receptor subunits. Cell surface expression of θ-myc or β2-myc was measured by a whole cell surface ELISA assay. The ELISA signal (A₆₂₀) is proportional to the amount of θ-myc or β2-myc at the cell surface. Background signal from mock-transfected control wells has been subtracted, and values are the mean ± SEM of triplicate determinations.