Table 1.
S. cerevisiae strains and plasmids used in this study
| Strain or plasmid | Descriptive name | Genotype or description | Reference or source |
|---|---|---|---|
| Strain | |||
| MBY41 | vps4Δvps2Δ | MBY3, VPS2::HIS3 | Babst et al. (2002) |
| EEY26-1 | vps4Δdid2Δ | MBY3, DID2::HIS3 | This study |
| MBY12 | vps4Δvps24Δ | MBY3, VPS24::HIS3 | Babst et al. (2002) |
| EEY17 | did2Δ | SEY6210, DID2::HIS3 | This study |
| JPY47 | vta1Δ | SEY6210.1, VTA1::HIS3 | Azmi et al. (2006) |
| EEY12 | vps4Δdid2Δ | MBY3, SNF7::HIS3 | Babst et al. (2002) |
| MBY63 | ist1Δ | SEY6210, IST1::HIS3 | This study |
| MCY2 | vta1Δist1Δ | SEY6210, VTA1::HIS3, IST1::HIS3 | This study |
| MCY3 | vps4Δist1Δ | MBY3, IST1::HIS3 | This study |
| CAY2 | did2Δist1Δ | EEY17, IST1:URA3 | This study |
| MBY3 | vps4Δ | SEY6210, VPS4::TRP1 | Babst et al. (1997) |
| MCY31 | did2Δ | W303, DID2::HIS3 | This study |
| W303 | WT | MATa leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 | Thomas and Rothstein (1989) |
| SEY6210 | WT | MATα leu2-3,112ura3-52his3-Δ200trp1-Δ901lys2-801suc2-Δ9 | Robinson et al. (1988) |
| Plasmids | |||
| pGO45 | GFP-CPS | URA3 ApR (pRS426) GFP-CPS1 | Odorizzi et al. (1998) |
| pCS24 | Ste2-GFP | URA3 ApR (pRS426) STE2-GFP | Odorizzi et al. (1998) |
| pMB241 | IST1-HA | URA3 ApR (pRS416) IST1-HA | This study |
| pMB243 | IST1-GFP | URA3 ApR (pRS416) IST1-GFP | This study |
| pMB287 | GST-IST1(CC) | ApR (pGEX-KG) GST-IST1(CC) | This study |
| pCD2 | GST-IST1 | ApR (pGEX-KG) GST-IST1 | This study |
| pCJ7 | IST1(ΔN)-GFP | URA3 ApR (pRS416) IST1(ΔN)-GFP | This study |
| pAH26 | 2 μDID2-HA | URA3 ApR (pRS425) DID2-HA | This study |
| pMB149 | vps4(E233Q)-HA | URA3 ApR (pRS416) vps4(E233Q)-HA | Babst et al. (2002) |
| pMB283 | VPS4-HA | URA3 ApR (pRS416) VPS4-HA | This study |
| pAH27 | DID2(ΔC58)-HA | LEU2 ApR (pRS425) DID2(ΔC58)-HA | This study |
| pAH25 | DID2(ΔC29)-HA | LEU2 ApR (pRS415) DID2(ΔC29)-HA | This study |
| pMB65 | vps4-ts | LEU2 ApR (pRS415) vps4-ts2–29 | This study |
| pMB286 | P(GAL1)-IST1 | TRP1 ApR (pRS414) P(GAL1)-IST1 | This study |
| pMB277 | P(GAL1)-IST1-HA | URA3 ApR (pRS416) P(GAL1)-IST1-HA | This study |