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. Author manuscript; available in PMC: 2008 Feb 5.
Published in final edited form as: Biochim Biophys Acta. 2007 Apr 10;1771(7):830–838. doi: 10.1016/j.bbalip.2007.04.001

Fig. 6.

Fig. 6

GPAT1 synthesized in vitro is targeted to mitochondria in a time and temperature dependent manner. Labeled recombinant GPAT1 was synthesized in vitro and incubated at 30 °C with pure mitochondria for different periods of time. Panel I: Mitochondrial proteins were separated on an 8% SDS-PAGE and autoradiographed. Panel II: Quantitative determination of the radioactivity incorporated into mitochondria. Results are expressed as radioactivity recovered from mitochondria and are representative of three independent experiments. The incorporation kinetics followed a hyperbolic-Hill-three-parameter equation with y = axb/(cb+xb), for the values: a = 4130, b = 0.72 and c = 5.95.