. Author manuscript; available in PMC: 2008 Feb 5.

Published in final edited form as: Biochim Biophys Acta. 2007 Apr 10;1771(7):830–838. doi: 10.1016/j.bbalip.2007.04.001

Table 1.

Fold enrichment of marker enzymes and GPAT activity compared to rat-liver homogenate

	Arylesterase	Cytochrome c oxidase	NEM-resistant GPAT	NEM-sensitive GPAT
Crude mitochondria	0.36±0.06	1	2.0±0.4	0.80±0.10
Microsomes	1.70±0.30	0.19±0.02	0.29±0.06	1.50±0.20
MAV	0.29±0.07	1.60±0.02	2.60±0.10	0.74±0.02
Pure mitochondria	ND	2.70±0.50	4.40±0.20	ND
MAM	1.20±0.40	ND	1.30±0.30	1.60±0.30

Cytochrome oxidase enrichment was normalized to crude mitochondria (0.045±0.015 U/mg). Results are shown as the means±SD from three independent experiments. Specific activities for the homogenate were: arylesterase 0.95±0.24 mmol/min mg, NEM-resistant GPAT 0.28±0.08 nmol/min mg and NEM-sensitive GPAT 0.40±0.06 nmol/min mg. ND: non-detectable values.