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. 2000 Oct 1;116(4):535–546. doi: 10.1085/jgp.116.4.535

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Modification by MTS reagents of K165C/C212S channels expressed in Xenopus oocytes. (A) Functional current induced by MTSEA. Repeated pulsing protocol 2, given one pulse every 2 s. Reagents applied as indicated by horizontal lines. (○) Current amplitudes measured at +40 mV. Examples of the current traces before and after MTSEA, and finally after dithiothreitol treatment are shown. Dotted lines are zero-current level. (B) Reduction of MTSEA-induced current after washout of the modifying reagent. Repeated pulsing protocol 1. Temperature, 20°C. (C) Side-chain structure of lysine and those of cysteine modified with various MTS reagents. (D) Modification of K165C by MTSET without generating functional channels. Pulsing protocol 1 used in this experiment. MTSES produced similar effects in blocking the current induction by MTSEA (data not shown). (E) Current induction in K165C channel by MTSPA. Dashed and solid curves represent the average of three recording traces before (control) and after the application of 0.2 mM MTSPA. Dotted line is zero-current level.