Figure 8.

Slow-gating behaviors of the heterodimer. (A–C) Slow gating of the heterodimer without MTSEA modification. (A) Single-channel trace at −60 mV revealed two current levels and several short inactivation events. (B) Voltage activation experiment to examine slow-gating transitions at the macroscopic current level (n = 3). (C) Temperature-jump experiment revealed prominent inactivation relaxation (n = 3). T₁ = 22.5°C, T₂ = 28.2°C. Symbols and experimental procedures for B and C were as in Fig. 2E and Fig. F. (D–F) Slow-gating behaviors of the MTSEA-modified heterodimers. (D) Single-channel recording of a MTSEA-modified heterodimer. Membrane potential, −60 mV. (E and F) Voltage and temperature jump experiments (n = 3). Procedures were as in B and C with 30–40 μM MTSEA in the bath. T₁ = 21.9°C, T₂ = 28.1°C.