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. 2000 Oct 1;116(4):507–520. doi: 10.1085/jgp.116.4.507

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Quantal secretory events detected in a dog pancreatic duct epithelial cell. (A) Representative amperometric signals. The cell was loaded in 70 mM serotonin solution and exocytotic events were monitored with a carbon-fiber electrode polarized at +600 mV in serotonin-free solution. (B) Quantal events from the same experiment plotted at higher time resolution. The arrows indicate “feet” preceding main spikes. The last event on the right is plotted on a different time scale. (C) Charge distribution histogram from the same experiment. Integrals of 105 individual amperometric spikes acquired without stimulation were analyzed. (D) Peak amplitude histogram of the same recording. Filter frequency: 200 Hz.