Table 1.
[Ca2+]i Elevations during Different Treatments
| Agent | Without BAPTA loading | After BAPTA loading | ||
|---|---|---|---|---|
| nM | n | nM | n | |
| Forskolin | 92 ± 25 | 11 | 5.5 ± 3.2 | 5 |
| Epinephrine | 1045 ± 283 | 7 | ND | |
| VIP | 82 ± 38 | 4 | ND | |
| 8-Br-cAMP | 447 ± 257 | 4 | ND | |
| PMA | 33 ± 23 | 4 | 5.3 ± 7.1 | 4 |
[Ca2+]i was measured in indo-1-AM–loaded cells. Resting [Ca2+]i was on average 67 ± 3 nM (n = 30). Peak [Ca2+]i was measured during forskolin (20 μM), epinephrine (1 μM), VIP (1 μM), 8-Br-cAMP (1 mM), or PMA (100 nM) treatments. Increase in [Ca2+]i above the resting levels after the treatment was analyzed.