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Linear diagram showing the approximate lengths of whole colicin Ia and the four carboxy-terminal fragments, CT-S, CT-M, CT-L, and CT-XL. The boundaries of the T, R, and C domains are from Wiener et al. 1997. The inter-domain regions are shaded and the full length of helix 1 is indicated. Mutant fragments have a cysteine residue near the amino terminus (453C/CT-S, 439C/CT-M, 326C/CT-L, and “−3” C/CT-XL), or not so near it (381C/CT-L). The amino-terminal His-tags are not shown.
