Figure 8.

C domain with its amino terminus anchored on the cis side by streptavidin made normal channels. A preincubated mixture containing 4 ng of biotinylated mutant 439C/CT-M without a His-tag and 150 ng of streptavidin was added to the cis compartment. The record shows two channels that opened and stayed in the higher-conductance state (37–38 pS) at +50 mV. The solution on both sides of the membrane and filtering were the same as in Fig. 5 B.